Intragenic HIV-1 env sequences that enhance gag expression

Expression of HIV-1 genes is regulated at multiple levels including the complex RNA splicing and transport mechanisms. Multiple cis-acting elements involved in these regulations have been previously identified in various regions of HIV-1 genome. Here we show that another cis-acting element was prese...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2003-04, Vol.309 (1), p.1-9
Main Authors: Suptawiwat, Ornpreya, Sutthent, Ruengpung, Lee, Tun-Hou, Auewarakul, Prasert
Format: Article
Language:English
Subjects:
Base Sequence
cis-acting element
Cloning, Molecular
DNA Primers
DNA, Viral - genetics
env
Expression
Gene Expression Regulation, Viral
Genes, env
Genes, gag - genetics
Genome, Viral
HIV-1
HIV-1 - genetics
Introns
Oligonucleotide Probes
Protein Biosynthesis
Restriction Mapping
Rev-dependent
RNA, Viral - genetics
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Summary:Expression of HIV-1 genes is regulated at multiple levels including the complex RNA splicing and transport mechanisms. Multiple cis-acting elements involved in these regulations have been previously identified in various regions of HIV-1 genome. Here we show that another cis-acting element was present in HIV-1 env region. This element enhanced the expression of Gag when inserted together with Rev response element (RRE) into a truncated HIV-1 genome in the presence of Rev. The enhancing activity was mapped to a 263-bp fragment in the gp41 region downstream to RRE. RNA analysis showed that it might function by promoting RNA stability and Rev-dependent RNA export. The enhancement was specific to Rev-dependent expression, since it did not enhance Gag expression driven by Sam68, a cellular protein that has been shown to be able to substitute for Rev in RNA export function.
ISSN:0042-6822
1096-0341
DOI:10.1016/S0042-6822(02)00084-3