Amperometric detection of perphenazine at a carbon fiber micro-disk bundle electrode by capillary zone electrophoresis

A simple method for determination of perphenazine by capillary zone electrophoresis with amperometric detection is described. The optimum conditions of separation and detection are 1.50×10 −3 mol/l Na 2B 4O 7−1.0×10 −3 mol/l NaOH (pH 9.9) for the buffer solution, 18 kV for the separation voltage, 5...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-06, Vol.789 (2), p.411-415
Main Authors: Liu, Daxing, Jin, Wenrui
Format: Article
Language:English
Subjects:
Analysis
Antipsychotic Agents - urine
Biological and medical sciences
Calibration
Carbon - chemistry
Electrochemistry - methods
Electrophoresis, Capillary - methods
General pharmacology
Humans
Medical sciences
Perphenazine
Perphenazine - urine
Pharmacology. Drug treatments
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Summary:A simple method for determination of perphenazine by capillary zone electrophoresis with amperometric detection is described. The optimum conditions of separation and detection are 1.50×10 −3 mol/l Na 2B 4O 7−1.0×10 −3 mol/l NaOH (pH 9.9) for the buffer solution, 18 kV for the separation voltage, 5 kV and 5 s for the injection voltage and the injection time, and 0.80 V versus saturated calomel electrode for the detection potential, respectively. The limit of detection is 5.0×10 −8 mol/l or 44 amol ( S/ N=3). The linear range of the calibration curve is 1.00×10 −7 to 1.00×10 −4 mol/l. The relative standard deviation is 1.5% for the migration time and 2.9% for the electrophoretic current at peak maximum. The method is applied to the determination of perphenazine in human urine.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(03)00074-6