Photoprotective properties of a hydrophilic extract of the fern Polypodium leucotomos on human skin cells

The effect of a hydrophilic extract of the fern Polypodium leucotomos (PLE) has been investigated in terms of photoprotection against UV-induced cell damage. PLE efficiently preserved human fibroblast survival and restored their proliferative capability when the cells were exposed to UVA light. This...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2003-04, Vol.70 (1), p.31-37
Main Authors: Alonso-Lebrero, José Luis, Domı&#x0301, nguez-Jiménez, Carmen, Tejedor, Reyes, Brieva, Aurora, Pivel, Juan Pablo
Format: Article
Language:English
Subjects:
Acetylcysteine - pharmacology
Ascorbic Acid - pharmacology
Cell Adhesion - drug effects
Cell Adhesion - radiation effects
Cell Division - drug effects
Cell Division - radiation effects
Cell Line
Cell Survival - drug effects
Cell Survival - radiation effects
Cells, Cultured
Chromans - pharmacology
Cytoskeleton
Cytoskeleton - drug effects
Cytoskeleton - radiation effects
Fibroblast
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - radiation effects
Humans
Keratinocyte
P. leucotomos
Photoprotection
Plant Extracts - pharmacology
Polypodium
Protective Agents - pharmacology
Skin - cytology
Skin - drug effects
Skin - radiation effects
Ultraviolet
Ultraviolet Rays
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Summary:The effect of a hydrophilic extract of the fern Polypodium leucotomos (PLE) has been investigated in terms of photoprotection against UV-induced cell damage. PLE efficiently preserved human fibroblast survival and restored their proliferative capability when the cells were exposed to UVA light. This effect was specific and dose-dependent. Photoprotection was not restricted to fibroblasts, as demonstrated by its effect on survival and proliferation of the human keratinocyte cell line HaCat. Finally, treatment of the cells with PLE prevented UV-induced morphological changes in human fibroblasts, namely disorganisation of F-actin-based cytoskeletal structures, coalescence of the tubulin cytoskeleton and mislocalization of adhesion molecules such as cadherins and integrins. Our in vitro results demonstrate the photoprotective effect of PLE on human cells and support its use in the preventive treatment of sunburning and skin pathologies associated with UV-mediated damage.
ISSN:1011-1344
1873-2682
DOI:10.1016/S1011-1344(03)00051-4