Ultrastructural localization of actin in normal human skin

Normal human skin was embedded in Lowicryl K4M. Actin microfilaments were localized by applying a postembedding immunogold technique using the monoclonal anti-actin antibody HHF35. Actin microfilaments are part of the cytoskeleton in muscle and nonmuscle cells. Together with myosin they produce cont...

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Bibliographic Details
Published in:Archives of Dermatological Research 1992, Vol.284 (4), p.242-245
Main Authors: METZLER, G, SCHAUMBURG-LEVER, G, FEHRENBACHER, B, MÖLLER, H
Format: Article
Language:English
Subjects:
Actins - analysis
Actins - immunology
Antibodies, Monoclonal - immunology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Humans
Skin - chemistry
Skin - ultrastructure
Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue
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Summary:Normal human skin was embedded in Lowicryl K4M. Actin microfilaments were localized by applying a postembedding immunogold technique using the monoclonal anti-actin antibody HHF35. Actin microfilaments are part of the cytoskeleton in muscle and nonmuscle cells. Together with myosin they produce contraction. The antibody labelled myofilaments in smooth muscle arrector pili cells, myoepithelial cells and pericytes. In sweat gland cells the microvilli system, a zone beneath the cytoplasma membrane corresponding to the adhesion belt region, and apocrine decapitation formations showed labelling.
ISSN:0340-3696
1432-069X
DOI:10.1007/BF00375802