Crosstalk between neurokinin receptors is relevant to hematopoietic regulation: cloning and characterization of neurokinin-2 promoter

• Published in: Journal of neuroimmunology 2003-05, Vol.138 (1), p.65-75
• Main Authors: Bandari, Persis S., Qian, Jing, Oh, Hyun S., Potian, Julius A., Yehia, Ghassan, Harrison, Jonathan S., Rameshwar, Pranela
• Format: Article
• Language: English
• Subjects: 5' Untranslated Regions - drug effects; Base Sequence; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Cell Division - physiology; Cells, Cultured; Cloning, Molecular - methods; Cytokines; Cytokines - pharmacology; Down-Regulation - drug effects; Gene Deletion; Hematopoiesis; Hematopoiesis - genetics; Hematopoiesis - physiology; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - metabolism; Humans; Molecular Sequence Data; Neuroimmunology; Neurokinin receptors; Neurokinin-1 Receptor Antagonists; Promoter Regions, Genetic - drug effects; Promoter Regions, Genetic - genetics; Receptor Cross-Talk - physiology; Receptors, Neurokinin-1 - genetics; Receptors, Neurokinin-1 - physiology; Receptors, Neurokinin-2 - antagonists & inhibitors; Receptors, Neurokinin-2 - chemistry; Receptors, Neurokinin-2 - genetics; Receptors, Neurokinin-2 - physiology; RNA, Messenger - metabolism; Stem cells; Stromal cells; Stromal Cells - drug effects; Stromal Cells - metabolism; Substance P - pharmacology; Transfection; Up-Regulation - drug effects
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/S0165-5728(03)00096-1

Neurokinin (NK)-1 and NK-2 receptors regulate hematopoiesis by interacting with neurotransmitters that belong to the tachykinin. This report studies the relationship between NK-1 and NK-2 in primary human bone marrow (BM) stroma, which supports hematopoiesis. Use of NK receptor antagonists and deficient stromal cells indicate that the neurotransmitter, substance P (SP), could exert dual hematopoietic effects (inhibitory or stimulatory), depending on the interacting receptor and crosstalk between NK-1 and NK-2. Cloning and identification of the minimal promoter for NK-2 and comparison with NK-1 promoter showed that the hematopoietic functions of NK receptors involve receptor crosstalk and the particular cytokine (IL-3, GM-CSF, TGF-β or IL-1α). Crosstalk between NK-1 and NK-2 adds to communication within neural–hematopoietic axis.