Effects of nicotine administration in developing muscle fibers of rats offspring

• Published in: Ecotoxicology and environmental safety 2003-06, Vol.55 (2), p.152-156
• Main Authors: Calore, E.E, da Rosa, A.R, Perez, N.M, Vilela De Almeida, L
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Desmin - metabolism; Female; Immunohistochemistry; Medical sciences; Muscle Fibers, Skeletal - drug effects; Muscle Fibers, Skeletal - metabolism; Muscle, Skeletal - cytology; Muscle, Skeletal - drug effects; Muscle, Skeletal - growth & development; Nicotine - pharmacology; Nicotinic Agonists - pharmacology; Pregnancy; Rats; Rats, Wistar; Receptors, Nicotinic - drug effects; Receptors, Nicotinic - metabolism; Tobacco, tobacco smoking; Toxicology; Vimentin - metabolism
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/S0147-6513(02)00118-5

The authors studied the effects of nicotine in developing skeletal muscles of rats. Pregnant Wistar rats received an enteral dose of nicotine (2.5, 5.0 or 10 mg/kg, respectively, in groups G2, G5, and G10) from the 10th to the 18th or 20th days of pregnancy. Myotube atrophy was observed mainly in 20-day-old fetuses of G10. Twenty-day-old control fetus and of G2 and G5 had a high sarcoplasmic expression of desmin (weaker in G10). Vimentin expression was similar in 18-day-old fetuses of the control, G2, and G5 groups, but it was weaker in 20-day-old fetuses of the G2 and G5 groups. This would indicate an acceleration of the maturation pattern of vimentin expression in these intoxicated fetus. In conclusion, high doses of nicotine induce myotube atrophy and decrease of the expression of intermediate filaments, whereas relatively low doses of nicotine (G2 and G5) induce an early decrease of vimentin expression with no myofiber atrophy.