Passage of vasoactive intestinal peptide across the blood–brain barrier

We investigated the ability of vasoactive intestinal peptide (VIP) to cross the blood–brain barrier (BBB), the interface between the peripheral circulation and central nervous system (CNS). VIP labeled with 131 I (I-VIP) and injected intravenously into mice was taken up by brain as determined by mul...

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Bibliographic Details
Published in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2003-03, Vol.24 (3), p.437-444
Main Authors: Dogrukol-Ak, Dilek, Banks, William A., Tuncel, Nese, Tuncel, Muzaffer
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood-Brain Barrier - physiology
Brain - metabolism
Buffers
Capillaries - physiology
Chromatography, High Pressure Liquid
Fundamental and applied biological sciences. Psychology
Injections, Intravenous
Iodine Radioisotopes
Male
Mice
Octanols
Protein Transport
Vasoactive Intestinal Peptide - administration & dosage
Vasoactive Intestinal Peptide - cerebrospinal fluid
Vasoactive Intestinal Peptide - metabolism
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Summary:We investigated the ability of vasoactive intestinal peptide (VIP) to cross the blood–brain barrier (BBB), the interface between the peripheral circulation and central nervous system (CNS). VIP labeled with 131 I (I-VIP) and injected intravenously into mice was taken up by brain as determined by multiple-time regression analysis. Excess unlabeled VIP was unable to impede the entry of I-VIP, indicating that passage is by nonsaturable transmembrane diffusion. High pressure liquid chromatography (HPLC) showed the radioactivity entering the brain to be intact I-VIP. After intracerebroventricular (i.c.v.) injection, I-VIP was sequestered by brain, slowing its efflux from the CNS. In summary, VIP crosses the BBB unidirectionally from blood to brain by transmembrane diffusion.
ISSN:0196-9781
1873-5169
DOI:10.1016/S0196-9781(03)00059-7