Comparison of hybrid and purebred in vitro-derived cattle embryos during in vitro culture

Frozen-thawed spermatozoa collected from a beef bull (Japanese Black) were used for in vitro fertilization (IVF) of matured oocytes obtained from dairy (Holstein) and beef (Japanese Black) females. Embryos were examined for fertilization, cleavage rate, interval between insemination and blastocyst p...

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Bibliographic Details
Published in:Animal reproduction science 2003-09, Vol.78 (1), p.1-11
Main Authors: Boediono, A., Suzuki, T., Godke, R.A.
Format: Article
Language:English
Subjects:
Animals
Blastocyst - physiology
Bovine
Breeding
Cattle - embryology
Cleavage Stage, Ovum
Coculture Techniques
Culture Techniques
Embryo
Embryonic and Fetal Development
Female
Fertilization in Vitro - veterinary
Hybridization, Genetic
In vitro development
In vitro fertilization
Male
Ovarian Follicle
Sex Ratio
Time Factors
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Summary:Frozen-thawed spermatozoa collected from a beef bull (Japanese Black) were used for in vitro fertilization (IVF) of matured oocytes obtained from dairy (Holstein) and beef (Japanese Black) females. Embryos were examined for fertilization, cleavage rate, interval between insemination and blastocyst production (experiment I), total cell number per embryo and sex ratio during blastocyst formation (experiment II), and blastocyst production rate of zygotes that developed to 2-, 4-, and 8-cell stages at 48 h post-fertilization (experiment III). Fertilized oocytes were cultured in vitro on a cumulus cell co-culture system. The fertilization and cleavage rate of oocytes groups were similar, however, the blastocyst production rate was greater ( P
ISSN:0378-4320
1873-2232
DOI:10.1016/S0378-4320(03)00065-4