Molecular characterization of a defensin in the IZD-MB-0503 cell line derived from immunocytes of the insect Mamestra brassicae (Lepidoptera)

The induction of anti-microbial peptides against Gram positive and negative bacteria in the IZD-MB-0503 cell line from the lepidopteran Mamestra brassicae is demonstrated, while no anti-fungal activity is detected. The identification of a defensin-like molecule active against Gram positive bacteria...

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Bibliographic Details
Published in:Biology of the cell 2003-01, Vol.95 (1), p.53-57
Main Authors: Mandrioli, Mauro, Bugli, Silvia, Saltini, Sabrina, Genedani, Susanna, Ottaviani, Enzo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cell Line
Defensin gene
Defensins - genetics
Defensins - immunology
Defensins - pharmacology
Genes, Insect
Immune response
IZD-MB-0503 cell line
Lepidoptera
Lepidoptera - immunology
Mamestra brassicae
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Homology, Amino Acid
Staphylococcus aureus - drug effects
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Summary:The induction of anti-microbial peptides against Gram positive and negative bacteria in the IZD-MB-0503 cell line from the lepidopteran Mamestra brassicae is demonstrated, while no anti-fungal activity is detected. The identification of a defensin-like molecule active against Gram positive bacteria is described for the first time in Lepidoptera. This molecule shows between 43% and 59% homology with group A defensins from other dipteran and hymenopteran species.
ISSN:0248-4900
1768-322X
DOI:10.1016/S0248-4900(02)01219-4