CTGF expression during mouse embryonic development

Connective tissue growth factor (CTGF) is a potent fibroblast mitogen and angiogenic factor which plays an important role in wound healing, cancerogenesis and fibrotic and vascular disease. Here we explored the regulation and the cellular site of the mRNA synthesis for this growth factor in the deve...

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Bibliographic Details
Published in:Cell and tissue research 2003-05, Vol.312 (2), p.175-188
Main Authors: Friedrichsen, Soenke, Heuer, Heike, Christ, Stephanie, Winckler, Miryam, Brauer, Daniel, Bauer, Karl, Raivich, Gennadij
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Bone and Bones - metabolism
Cardiovascular System - metabolism
Cartilage - metabolism
Connective Tissue Growth Factor
Female
Gene Expression Regulation, Developmental - physiology
Image Processing, Computer-Assisted
Immediate-Early Proteins - biosynthesis
In Situ Hybridization
Intercellular Signaling Peptides and Proteins - biosynthesis
Mice
Pregnancy
RNA Probes
RNA, Messenger - biosynthesis
Tissue Distribution
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Summary:Connective tissue growth factor (CTGF) is a potent fibroblast mitogen and angiogenic factor which plays an important role in wound healing, cancerogenesis and fibrotic and vascular disease. Here we explored the regulation and the cellular site of the mRNA synthesis for this growth factor in the developing mouse embryo by in situ hybridisation. Strong and persistent CTGF gene expression was limited to three types of tissue: the vascular endothelium, particularly the high-pressure part of the cardiovascular system, condensed connective tissue around bone and cartilage, and maturing layer VII neurons in the cerebral cortex. With few exceptions (late tooth bud, neuroepithelium) epithelial tissue was negative. Very transient but strong expression was observed early during formation of cartilage, in late stages during perichondral ossification, on cerebral neuroepithelium, and in several discrete stages of tooth formation, on mesenchymal precursors of odontoblasts condensing on inner dental epithelium, and later on apposing regions of ameloblast and odontoblast epithelium. Altogether, the current study suggests that CTGF performs a dual role: a continuous function in the cardiovascular system, bone and cartilage-associated mesenchyme and maturing layer VII neurons, but also a more transient function associated with the formation of cartilage, bone, tooth and cerebral nerve cells.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-003-0712-6