Reducing the overlap problem in the proton NMR spectra of oligosaccharides by application of pseudo-four-dimensional homonuclear HOHAHA-HOHAHA-COSY

A new homonuclear NMR experiment is described for the assignment of the proton NMR spectra of oligosaccharides, namely HOHAHA-HOHAHA-COSY (where HOHAHA is homonuclear Hartmann-Hahn spectroscopy and COSY is correlated spectroscopy). While this technique may formally be thought of as a four-dimensiona...

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Bibliographic Details
Published in:Glycobiology (Oxford) 1992-08, Vol.2 (4), p.293-298
Main Authors: Rutherford, T.J., Homans, S.W.
Format: Article
Language:English
Subjects:
Carbohydrate Conformation
Carbohydrate Sequence
four-dimensional NMR
Magnetic Resonance Spectroscopy - methods
Molecular Sequence Data
N-linked glycan
Oligosaccharides - chemistry
serotransferrin
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Summary:A new homonuclear NMR experiment is described for the assignment of the proton NMR spectra of oligosaccharides, namely HOHAHA-HOHAHA-COSY (where HOHAHA is homonuclear Hartmann-Hahn spectroscopy and COSY is correlated spectroscopy). While this technique may formally be thought of as a four-dimensional NMR experiment, by use of selective pulses it is demonstrated that the analogous pseudo-four-dimensional experiment is a valuable improvement over conventional three-dimensional HOHAHA-COSY, in that the degree of resonance overlap is markedly reduced by the dispersion of resonances into a fourth effective dimension. The technique is demonstrated by application to the biantennary nonasaccharide Galβ1-4-GlcNAcβ1-2Manα1-6(Galβ1-4GlcNAcβ1-2Manα1-3)Manβ1-4GlcNacβ1-4GlcNac
ISSN:0959-6658
1460-2423
DOI:10.1093/glycob/2.4.293