Ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads

A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme a...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1992, Vol.32 (1-3), p.73-78
Main Authors: Marques, E.T.A. Jr, Filho, J.L.L
Format: Article
Language:English
Subjects:
Alkylation
amines
Amines - chemistry
ascorbate oxidase
Ascorbate Oxidase - chemistry
ascorbic acid
Ascorbic Acid - chemistry
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Cucurbita maxima
electrodes
Enzymes, Immobilized
fruit juices
Fundamental and applied biological sciences. Psychology
glass
immobilization
Methods. Procedures. Technologies
Microspheres
Various methods and equipments
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Description
Summary:A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protection. A low-cost, home-made oxygen electrode was applied as a transducer. The system has sensitivity from 62.5 up to 500 microM of ascorbic acid with satisfactory operation for more than 2 mo.
ISSN:0273-2289
1559-0291
DOI:10.1007/bf02922149