The cruciferin gene family in radish

In order to analyse the cruciferin gene family in radish a cDNA library was screened either with heterologous rapeseed probes or by differential screening and sequencing. We could identify six partial cDNA clones belonging to two different groups of cruciferin genes which do not cross-hybridize, and...

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Bibliographic Details
Published in:Plant molecular biology 1992-11, Vol.20 (3), p.467-479
Main Authors: Depigny-This, D. (Perpignan Univ. (France). Lab. de Physiologie et Biologie Moleculaire Vegetales), Raynal, M, Aspart, L, Delseny, M, Grellet, F
Format: Article
Language:English
Subjects:
ADN
Allergens
Amino Acid Sequence
Antigens, Plant
Arabidopsis - genetics
Base Sequence
Biological and medical sciences
Brassica - genetics
Cloning, Molecular
DNA
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE
GENE EXPRESSION
Gene Library
GENES
Genes, Plant - genetics
Genes. Genome
GRAINE
Introns - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Multigene Family - genetics
Plant Proteins - genetics
Plants - genetics
PROTEINAS
PROTEINE
PROTEINS
RAPHANUS SATIVUS
Regulatory Sequences, Nucleic Acid - genetics
RNA, Messenger - analysis
Seed Storage Proteins
SEEDS
Seeds - genetics
SEMILLA
Sequence Homology, Amino Acid
Time Factors
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Summary:In order to analyse the cruciferin gene family in radish a cDNA library was screened either with heterologous rapeseed probes or by differential screening and sequencing. We could identify six partial cDNA clones belonging to two different groups of cruciferin genes which do not cross-hybridize, and probably three distinct subfamilies. One of these classes corresponds to the previously described cruciferin from rapeseed and Arabidopsis. A gene corresponding to the second group, as well as its border sequences, was isolated from a radish genomic library and analysed in more detail. The cruciferin gene (cruRS) contains three introns and encodes a 479 amino acid protein. The transcription initiation site was determined. The expression of the different group of genes was studied by northern blot analysis: genes of both classes are expressed simultaneously and roughly at the same level between 25 and 35 days after flowering. Cruciferin gene copy number was estimated by Southern blot analysis. There appear to be seven or eight genes in one class and three in the other, located at different loci.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00040606