Mucosal mast cells and nematode infection: strain-specific differences in mast cell precursor frequency revisited

Mucosal mast cells (MMC) play an important role in the immune response against selected species of intestinal nematode. The kinetics with which different strains of inbred mice resolve infection with Trichinella spiralis correlates with their ability to mount MMC responses in the intestinal mucosa....

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Published in:Journal of helminthology 2003-06, Vol.77 (2), p.155-161
Main Authors: Brown, J.K., Wright, S.H., Miller, H.R.P.
Format: Article
Language:English
Subjects:
Animals
Antibodies - analysis
Bone Marrow Cells
Cell Differentiation
Cells, Cultured
Chymases
Culture Media
Fluorescent Antibody Technique
Interleukin-3
Interleukin-9
Intestinal Diseases, Parasitic - immunology
Intestinal Mucosa - immunology
Male
Mast Cells - immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Serine Endopeptidases - immunology
Species Specificity
Stem Cell Factor
Transforming Growth Factor beta
Trichinella spiralis
Trichinellosis - immunology
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cited_by cdi_FETCH-LOGICAL-c451t-3fba6eb6e190fd59931c538c1f6b47464bc7fc5c346313664f525c51d511d4683
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container_title Journal of helminthology
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creator Brown, J.K.
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Miller, H.R.P.
description Mucosal mast cells (MMC) play an important role in the immune response against selected species of intestinal nematode. The kinetics with which different strains of inbred mice resolve infection with Trichinella spiralis correlates with their ability to mount MMC responses in the intestinal mucosa. Homologues of MMC that express and constitutively secrete abundant amounts of the granule chymase, mouse mast cell protease-1 (mMCP-1), can be generated in vitro from bone marrow cultures supplemented with interleukins-3 and -9, stem cell factor and transforming growth factor-β1. Using the enhanced growth characteristics of these MMC homologues, a novel limiting dilution assay for mast cell precursor (MCp) frequency has been developed. The assay is highly specific, in that cultures containing mast cells are identified with mMCP-1 specific antibody, and almost three-fold more sensitive than previously published systems. MCp frequencies were compared in BALB/c and C57/BL10 strains of mice that, respectively, respond rapidly and slowly to infection with T. spiralis. MCp frequency (1/378 bone marrow cells) was significantly greater (P
doi_str_mv 10.1079/JOH2002160
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source Cambridge University Press
subjects Animals
Antibodies - analysis
Bone Marrow Cells
Cell Differentiation
Cells, Cultured
Chymases
Culture Media
Fluorescent Antibody Technique
Interleukin-3
Interleukin-9
Intestinal Diseases, Parasitic - immunology
Intestinal Mucosa - immunology
Male
Mast Cells - immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Serine Endopeptidases - immunology
Species Specificity
Stem Cell Factor
Transforming Growth Factor beta
Trichinella spiralis
Trichinellosis - immunology
title Mucosal mast cells and nematode infection: strain-specific differences in mast cell precursor frequency revisited
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