Effect of perturbing diacylglycerol metabolism on cytosolic free Ca2+ oscillations induced in single hepatocytes

Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca2+‐mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vas...

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Bibliographic Details
Published in:FEBS letters 1992-11, Vol.312 (1), p.57-60
Main Authors: Sanchez-Bueno, Antonio, Cobbold, Peter H.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Ca2+ oscillation
Calcium - metabolism
Cell physiology
Cells, Cultured
Cytosol - metabolism
cytosolic concentration of free Ca2
Diacylglycerol
Diacylglycerol Kinase
diC8
Diglycerides - metabolism
free Ca
Fundamental and applied biological sciences. Psychology
Kinetics
Liver - drug effects
Liver - metabolism
Male
Molecular and cellular biology
Oscillometry
Phenylephrine - pharmacology
Phosphotransferases - antagonists & inhibitors
Pyrimidinones - pharmacology
R 59 022
Rats
Rats, Wistar
Signal transduction
Single hepatocyte
sn-1,2-diacylglycerol
sn-1,2-dioctanoylglycerol
Thiazoles - pharmacology
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Summary:Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca2+‐mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These results agree with a negative feedback effect of protein kinase C on free Ca oscillations.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)81409-F