Molecular cloning and developmental expression of a human kidney S-adenosylmethionine synthetase

cDNA clones encoding the human kidney S-adenosylmethionine synthetase (kidney-type isozyme) were isolated. The amino acid sequence deduced from the cDNA indicates that this enzyme contains 395 amino acids and has a molecular mass of 43,660 Da. The predicted amino acid sequence of this protein shares...

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Bibliographic Details
Published in:FEBS letters 1992-11, Vol.312 (1), p.37-41
Main Authors: Horikawa, Saburo, Tsukada, Kinji
Format: Article
Language:English
Subjects:
Adult
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
cDNA
cDNA sequence
Cloning, Molecular
DNA - genetics
DNA - isolation & purification
Fetus
Fundamental and applied biological sciences. Psychology
Gene expression
genes
Human kidney
Humans
Isoenzymes - genetics
Isozyme
kidney
Kidney - embryology
Kidney - enzymology
Liver - enzymology
man
methionine adenosyltransferase
Methionine Adenosyltransferase - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Molecular Weight
nucleotide sequence
Polymerase Chain Reaction - methods
predictions
Rats
RNA, Messenger - metabolism
S-Adenosylmethionine
Sequence Homology, Amino Acid
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Summary:cDNA clones encoding the human kidney S-adenosylmethionine synthetase (kidney-type isozyme) were isolated. The amino acid sequence deduced from the cDNA indicates that this enzyme contains 395 amino acids and has a molecular mass of 43,660 Da. The predicted amino acid sequence of this protein shares 84% similarity with that of human liver S-adenosylmethionine synthetase (liver-type isozyme). In addition, the developmental expression of these two isozyme mRNAs has been studied in the human liver using the reverse transcription—polymerase chain reaction (RT-PCR).
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)81405-B