Heterogeneous pattern of chromosomal breakpoints involving the MYC locus in multiple myeloma

Chromosomal rearrangements of the MYC locus, which often involve the IG loci, are recurrent events in multiple myeloma (MM) and plasma cell leukemia (PCL). We used dual‐color fluorescence in situ hybridization (FISH) to characterize the breakpoint locations of chromosomal translocations/rearrangemen...

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Bibliographic Details
Published in:Genes chromosomes & cancer 2003-07, Vol.37 (3), p.261-269
Main Authors: Fabris, Sonia, Storlazzi, Clelia Tiziana, Baldini, Luca, Nobili, Lucia, Lombardi, Luigia, Maiolo, Anna Teresa, Rocchi, Mariano, Neri, Antonino
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Cell Line, Transformed
Chromosome Breakage - genetics
Chromosomes, Human, Pair 8 - genetics
Female
Gene Rearrangement - genetics
Genes, myc - genetics
Genetic Heterogeneity
Genetic Markers - genetics
Humans
In Situ Hybridization, Fluorescence
Male
Middle Aged
Multiple Myeloma - genetics
Multiple Myeloma - pathology
Oncogene Proteins, Fusion - genetics
Translocation, Genetic - genetics
Tumor Cells, Cultured
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Summary:Chromosomal rearrangements of the MYC locus, which often involve the IG loci, are recurrent events in multiple myeloma (MM) and plasma cell leukemia (PCL). We used dual‐color fluorescence in situ hybridization (FISH) to characterize the breakpoint locations of chromosomal translocations/rearrangements involving the MYC locus at 8q24 found in a panel of 14 MM cell lines and 70 primary tumors (66 MM and 4 PCL). MYC locus alterations were observed in 21 cases: MYC/IG (mainly IGH@) fusions in 11 cell lines and three patients (2 MM and 1 PCL), and extra signals and/or abnormal MYC localizations in seven patients (5 MM and 2 PCL). Fourteen of these cases were investigated by FISH analyses by use of a panel of BAC clones covering about 6 Mb encompassing the MYC locus. The breakpoints were localized in a region 100–250 kb centromeric to MYC in four cases, a region 500–800 kb telomeric to the gene in four cases, and regions ≥ 2 Mb centromeric or telomeric to MYC in five cases. Two different breakpoints were detected in the KMS‐18 cell line, whereas the insertion of a MYC allele was found in a complex t(16;22) chromosomal translocation in the RPMI8226 cell line. Our data document a relatively high dispersion of 8q24 breakpoints in MM. © 2003 Wiley‐Liss, Inc.
ISSN:1045-2257
1098-2264
DOI:10.1002/gcc.10211