Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1

A method was developed to determine the conditional association constants of cadmium(II) [Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity...

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Bibliographic Details
Published in:Analytical biochemistry 2010-07, Vol.402 (2), p.200-202
Main Authors: Ogawa, Shinya, Yoshimura, Etsuro
Format: Article
Language:English
Subjects:
Acetates
Association constant
Cadmium
Cadmium - chemistry
Coordination Complexes - chemistry
Fluorescence
FluoZin-1
Glutathione
Glutathione - chemistry
Thermodynamics
Titrimetry
Xanthenes
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Summary:A method was developed to determine the conditional association constants of cadmium(II) [Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity of FluoZin-1 to estimate levels of free Cd(II). The results were analyzed with a nonlinear least-squares method using the Solver algorithm of Microsoft Excel to yield conditional association constants for 1:1 and 1:2 Cd(II)–glutathione complexes. The values obtained were consistent with those reported previously using isothermal titration calorimetry.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2010.03.030