Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1

A method was developed to determine the conditional association constants of cadmium(II) [Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 2010-07, Vol.402 (2), p.200-202
Main Authors: Ogawa, Shinya, Yoshimura, Etsuro
Format: Article
Language:English
Subjects:
Acetates
Association constant
Cadmium
Cadmium - chemistry
Coordination Complexes - chemistry
Fluorescence
FluoZin-1
Glutathione
Glutathione - chemistry
Thermodynamics
Titrimetry
Xanthenes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c415t-b6eb7fabf0b89ee55d5ee82259e241851b7a3af8b163fa8c4b8434cbdaede40a3
cites cdi_FETCH-LOGICAL-c415t-b6eb7fabf0b89ee55d5ee82259e241851b7a3af8b163fa8c4b8434cbdaede40a3
container_end_page 202
container_issue 2
container_start_page 200
container_title Analytical biochemistry
container_volume 402
creator Ogawa, Shinya
Yoshimura, Etsuro
description A method was developed to determine the conditional association constants of cadmium(II) [Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity of FluoZin-1 to estimate levels of free Cd(II). The results were analyzed with a nonlinear least-squares method using the Solver algorithm of Microsoft Excel to yield conditional association constants for 1:1 and 1:2 Cd(II)–glutathione complexes. The values obtained were consistent with those reported previously using isothermal titration calorimetry.
doi_str_mv 10.1016/j.ab.2010.03.030
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733090776</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003269710001983</els_id><sourcerecordid>733090776</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-b6eb7fabf0b89ee55d5ee82259e241851b7a3af8b163fa8c4b8434cbdaede40a3</originalsourceid><addsrcrecordid>eNp1UD1v2zAQJYoEtZN271RoSzrIOYr67BY4dWMgQJZ26UIcqZNNQxJdkgoQ5M-HgtNsBQ44vuN7D3ePsS8cVhx4eXNYoVplECGIWPCBLTk0ZRqfzRlbAoBIs7KpFuzC-wMA53lRfmSLDERelRUs2csdBXKDGTEYOya2S8I-Yts-jzgYnRzR4TBT_PynsR3MNFxvt98S9N5qc5IFm-z6KWDYR0TJ5M24m42Srp-sI69pDIkj3M19E2d_zJjyT-y8w97T57d-yX5vfvxa36cPjz-369uHVOe8CKkqSVUdqg5U3RAVRVsQ1VlWNJTlvC64qlBgVyteig5rnas6F7lWLVJLOaC4ZFcn36OzfyfyQQ4mrtT3OJKdvKxEjAuqqoxMODG1s9476uTRmQHds-Qg58TlQaKSc-ISRCyIkq9v5pMaqH0X_Is4Er6fCBRPfDLkpNeGRk2tcaSDbK35v_sr7KuTUw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733090776</pqid></control><display><type>article</type><title>Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1</title><source>ScienceDirect Freedom Collection</source><creator>Ogawa, Shinya ; Yoshimura, Etsuro</creator><creatorcontrib>Ogawa, Shinya ; Yoshimura, Etsuro</creatorcontrib><description>A method was developed to determine the conditional association constants of cadmium(II) [Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity of FluoZin-1 to estimate levels of free Cd(II). The results were analyzed with a nonlinear least-squares method using the Solver algorithm of Microsoft Excel to yield conditional association constants for 1:1 and 1:2 Cd(II)–glutathione complexes. The values obtained were consistent with those reported previously using isothermal titration calorimetry.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2010.03.030</identifier><identifier>PMID: 20347670</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetates ; Association constant ; Cadmium ; Cadmium - chemistry ; Coordination Complexes - chemistry ; Fluorescence ; FluoZin-1 ; Glutathione ; Glutathione - chemistry ; Thermodynamics ; Titrimetry ; Xanthenes</subject><ispartof>Analytical biochemistry, 2010-07, Vol.402 (2), p.200-202</ispartof><rights>2010 Elsevier Inc.</rights><rights>2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-b6eb7fabf0b89ee55d5ee82259e241851b7a3af8b163fa8c4b8434cbdaede40a3</citedby><cites>FETCH-LOGICAL-c415t-b6eb7fabf0b89ee55d5ee82259e241851b7a3af8b163fa8c4b8434cbdaede40a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20347670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ogawa, Shinya</creatorcontrib><creatorcontrib>Yoshimura, Etsuro</creatorcontrib><title>Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A method was developed to determine the conditional association constants of cadmium(II) [Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity of FluoZin-1 to estimate levels of free Cd(II). The results were analyzed with a nonlinear least-squares method using the Solver algorithm of Microsoft Excel to yield conditional association constants for 1:1 and 1:2 Cd(II)–glutathione complexes. The values obtained were consistent with those reported previously using isothermal titration calorimetry.</description><subject>Acetates</subject><subject>Association constant</subject><subject>Cadmium</subject><subject>Cadmium - chemistry</subject><subject>Coordination Complexes - chemistry</subject><subject>Fluorescence</subject><subject>FluoZin-1</subject><subject>Glutathione</subject><subject>Glutathione - chemistry</subject><subject>Thermodynamics</subject><subject>Titrimetry</subject><subject>Xanthenes</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp1UD1v2zAQJYoEtZN271RoSzrIOYr67BY4dWMgQJZ26UIcqZNNQxJdkgoQ5M-HgtNsBQ44vuN7D3ePsS8cVhx4eXNYoVplECGIWPCBLTk0ZRqfzRlbAoBIs7KpFuzC-wMA53lRfmSLDERelRUs2csdBXKDGTEYOya2S8I-Yts-jzgYnRzR4TBT_PynsR3MNFxvt98S9N5qc5IFm-z6KWDYR0TJ5M24m42Srp-sI69pDIkj3M19E2d_zJjyT-y8w97T57d-yX5vfvxa36cPjz-369uHVOe8CKkqSVUdqg5U3RAVRVsQ1VlWNJTlvC64qlBgVyteig5rnas6F7lWLVJLOaC4ZFcn36OzfyfyQQ4mrtT3OJKdvKxEjAuqqoxMODG1s9476uTRmQHds-Qg58TlQaKSc-ISRCyIkq9v5pMaqH0X_Is4Er6fCBRPfDLkpNeGRk2tcaSDbK35v_sr7KuTUw</recordid><startdate>20100715</startdate><enddate>20100715</enddate><creator>Ogawa, Shinya</creator><creator>Yoshimura, Etsuro</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100715</creationdate><title>Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1</title><author>Ogawa, Shinya ; Yoshimura, Etsuro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-b6eb7fabf0b89ee55d5ee82259e241851b7a3af8b163fa8c4b8434cbdaede40a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acetates</topic><topic>Association constant</topic><topic>Cadmium</topic><topic>Cadmium - chemistry</topic><topic>Coordination Complexes - chemistry</topic><topic>Fluorescence</topic><topic>FluoZin-1</topic><topic>Glutathione</topic><topic>Glutathione - chemistry</topic><topic>Thermodynamics</topic><topic>Titrimetry</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ogawa, Shinya</creatorcontrib><creatorcontrib>Yoshimura, Etsuro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogawa, Shinya</au><au>Yoshimura, Etsuro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2010-07-15</date><risdate>2010</risdate><volume>402</volume><issue>2</issue><spage>200</spage><epage>202</epage><pages>200-202</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A method was developed to determine the conditional association constants of cadmium(II) [Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity of FluoZin-1 to estimate levels of free Cd(II). The results were analyzed with a nonlinear least-squares method using the Solver algorithm of Microsoft Excel to yield conditional association constants for 1:1 and 1:2 Cd(II)–glutathione complexes. The values obtained were consistent with those reported previously using isothermal titration calorimetry.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>20347670</pmid><doi>10.1016/j.ab.2010.03.030</doi><tpages>3</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0003-2697
ispartof Analytical biochemistry, 2010-07, Vol.402 (2), p.200-202
issn 0003-2697
1096-0309
language eng
recordid cdi_proquest_miscellaneous_733090776
source ScienceDirect Freedom Collection
subjects Acetates
Association constant
Cadmium
Cadmium - chemistry
Coordination Complexes - chemistry
Fluorescence
FluoZin-1
Glutathione
Glutathione - chemistry
Thermodynamics
Titrimetry
Xanthenes
title Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T13%3A04%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20thermodynamic%20parameters%20of%20cadmium(II)%20association%20to%20glutathione%20using%20the%20fluorescent%20reagent%20FluoZin-1&rft.jtitle=Analytical%20biochemistry&rft.au=Ogawa,%20Shinya&rft.date=2010-07-15&rft.volume=402&rft.issue=2&rft.spage=200&rft.epage=202&rft.pages=200-202&rft.issn=0003-2697&rft.eissn=1096-0309&rft_id=info:doi/10.1016/j.ab.2010.03.030&rft_dat=%3Cproquest_cross%3E733090776%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c415t-b6eb7fabf0b89ee55d5ee82259e241851b7a3af8b163fa8c4b8434cbdaede40a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=733090776&rft_id=info:pmid/20347670&rfr_iscdi=true