Differential proteomic characterization between normal peritoneal fluid and diabetic peritoneal dialysate

• Published in: Nephrology, dialysis, transplantation dialysis, transplantation, 2010-06, Vol.25 (6), p.1955-1963
• Main Authors: Wang, Hsien-Yi, Tian, Yu-Feng, Chien, Chih-Chiang, Kan, Wei-Chih, Liao, Pao-Chi, Wu, Hsin-Yi, Su, Shih-Bin, Lin, Ching-Yih
• Format: Article
• Language: English
• Subjects: Adult; Aged; alpha-Macroglobulins - analysis; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Ascitic Fluid - chemistry; Associated diseases and complications; Biological and medical sciences; Biomarkers - analysis; Blotting, Western; Complement C4a - analysis; diabetes mellitus; Diabetes. Impaired glucose tolerance; Diabetic Nephropathies - metabolism; Diabetic Nephropathies - therapy; Dialysis Solutions - chemistry; Electrophoresis, Gel, Two-Dimensional; Emergency and intensive care: renal failure. Dialysis management; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Female; Haptoglobins - analysis; Humans; Immunoglobulins - analysis; Intensive care medicine; Male; Medical sciences; Middle Aged; peritoneal dialysate; peritoneal dialysis; Peritoneal Dialysis - adverse effects; Peritoneum - chemistry; Peritoneum - injuries; Proteome - analysis; proteomic analysis; Proteomics; Tandem Mass Spectrometry; two-dimensional gel electrophoresis; Vitamin D-Binding Protein - analysis
• ISSN: 0931-0509; 1460-2385
• DOI: 10.1093/ndt/gfp696

Background. Since the mechanism of comorbidity and mortality in peritoneal dialysis is unclear, a comparison of peritoneal dialysate and normal peritoneal fluid may provide clues to the biological and pathological processes involved in peritoneal damage. Methods. Peritoneal dialysate and control samples were collected from five diabetes mellitus (DM) patients and two patients receiving laparoscopic cholecystectomy. Proteins were separated by two-dimensional gel electrophoresis (2D-GE). After image analysis, altered gel spots between these two sample groups were subjected to tryptic digestion and mass spectrometry analysis. The results were searched against the NCBI database. Results. A total of 26 protein spots were considered altered in 2D-GE between the two sample groups. After western blotting confirmation, vitamin D-binding protein, haptoglobin and α-2-microglobulin were at higher levels in the DM samples, while complement C4-A and IGK@ protein were at lower levels compared to the control samples. Conclusion. The loss of vitamin D-binding protein, haptoglobin and α-2-microglobulin may be due to a change in the permeability of the peritoneal membrane to middle-sized proteins or leakage from peritoneal inflammation. Lower levels of complement C4-A in dialysate may shed light on the beginning of peritoneal membrane scleroses.