In vivo multimodal nonlinear optical imaging of mucosal tissue

We present a multimodal nonlinear imaging approach to elucidate microstructures and spectroscopic features of oral mucosa and submucosa in vivo. The hamster buccal pouch was imaged using 3-D high resolution multiphoton and second harmonic generation microscopy. The multimodal imaging approach enable...

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Bibliographic Details
Published in:Optics express 2004-05, Vol.12 (11), p.2478-2486
Main Authors: Sun, Ju, Shilagard, Tuya, Bell, Brent, Motamedi, Massoud, Vargas, Gracie
Format: Article
Language:English
Online Access:Get full text
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Summary:We present a multimodal nonlinear imaging approach to elucidate microstructures and spectroscopic features of oral mucosa and submucosa in vivo. The hamster buccal pouch was imaged using 3-D high resolution multiphoton and second harmonic generation microscopy. The multimodal imaging approach enables colocalization and differentiation of prominent known spectroscopic and structural features such as keratin, epithelial cells, and submucosal collagen at various depths in tissue. Visualization of cellular morphology and epithelial thickness are in excellent agreement with histological observations. These results suggest that multimodal nonlinear optical microscopy can be an effective tool for studying the physiology and pathology of mucosal tissue.
ISSN:1094-4087
DOI:10.1364/OPEX.12.002478