Shedding of gACE from residual body membrane of rat sperm

Abstract Germinal angiotensin I-converting enzyme (gACE) is expressed only in the testis and is uniquely present in developing spermatids and sperm. We previously purified soluble gACE from porcine seminal plasma, and reported that gACE was secreted from residual body on spermatozoa by the other pep...

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Bibliographic Details
Published in:Legal medicine (Tokyo, Japan) Japan), 2009-04, Vol.11, p.S309-S310
Main Authors: Takeuchi, Keisuke, Sakaue, Tomohisa, Yamamoto, Yoshio, Nishi, Katsuji, Ohkubo, Iwao
Format: Article
Language:English
Subjects:
Animals
Antipain - pharmacology
Cell Membrane - metabolism
Edetic Acid - pharmacology
Epididymis - metabolism
gACE
Internal Medicine
Male
Peptidyl-Dipeptidase A - metabolism
Protease Inhibitors - pharmacology
Rats
Sheddase
Spermatids - metabolism
Spermatozoa - metabolism
Sulfones - pharmacology
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Summary:Abstract Germinal angiotensin I-converting enzyme (gACE) is expressed only in the testis and is uniquely present in developing spermatids and sperm. We previously purified soluble gACE from porcine seminal plasma, and reported that gACE was secreted from residual body on spermatozoa by the other peptidase(s), namely Sheddase. Using Nma/DNP substrate, it was observed that the shedding activity in testicular fluid was stronger than in the sperm membrane and epididymal fluid. The shedding activity was inhibited by AEBSF and antipain, and not by EDTA and E-64. Accordingly, it is thought that Sheddase is an endo-type of serine peptidase.
ISSN:1344-6223
1873-4162
DOI:10.1016/j.legalmed.2009.02.074