Effects of Ergothioneine from Mushrooms (Flammulina velutipes) on Melanosis and Lipid Oxidation of Kuruma Shrimp (Marsupenaeus japonicus)

• Published in: Journal of agricultural and food chemistry 2010-02, Vol.58 (4), p.2577-2585
• Main Authors: Encarnacion, Angel B, Fagutao, Fernand, Hirono, Ikuo, Ushio, Hideki, Ohshima, Toshiaki
• Format: Article
• Language: English
• Subjects: Agaricales - chemistry; animal feeding; Animals; antimelanosic properties; antioxidant activity; betaine; Biological and medical sciences; Catechol Oxidase - metabolism; dietary supplements; DNA Primers; ergothioneine; Ergothioneine - metabolism; Fish and seafood industries; Flammulina - chemistry; Flammulina velutipes; Food Chemistry/Biochemistry; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; gene expression; Hemolymph - enzymology; lipid peroxidation; Lipid Peroxidation - drug effects; Lipid Peroxides - analysis; Lipid Peroxides - metabolism; Marsupenaeus japonicus; melanosis; Melanosis - veterinary; mushrooms; Penaeidae - drug effects; Penaeidae - physiology; Plant Extracts - pharmacology; Reverse Transcriptase Polymerase Chain Reaction; shrimp
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf903944y

The antimelanosic and antioxidative properties of a hot water extract prepared from the fruiting body of the edible mushroom (Flammulina velutipes) were evaluated by dietary supplementation in Kuruma shrimp (Marsupenaeus japonicus) for possible aquaculture application. The extract contained ergothioneine (ERT) at a level of 2.05 mg/mL. A commercial standard of l-ergothioneine (l-ERT) and the mushroom extract showed inhibitory activity against mushroom polyphenoloxidase (PPO). Feeding of the extract had no adverse effects on the immune systems of the shrimp under the present experimental conditions. Supplementation of the extract in the diet significantly suppressed PPO activities in the hemolymphs of the shrimp. Expression of the prophenoloxidase (proPO) gene decreased in the hemocyte of the Kuruma shrimp fed with the mushroom extract. Consequently, development of melanosis was significantly suppressed in the supplement fed shrimp during ice storage. Lipid oxidation was also effectively controlled in the supplement fed group throughout the storage period. In vitro experiments showed that l-ERT effectively inhibited the activation of proPO in the hemocyte lysate supernatant (HLS). The transcript of the proPO gene in the hemocyte showed lower expression in the l-ERT-treated HLS. It was concluded that dietary supplementation of the mushroom extract in shrimp could be a promising approach to control post mortem development of melanosis and lipid oxidation in shrimp muscles.