Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors

The stromal cell–derived factor (SDF)‐1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution to postnatal myogenesis and the role of the alternate SDF‐1 receptor, CXC receptor 7 (CXCR7), are poorly characterized. Western blot analysis and rea...

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Bibliographic Details
Published in:Muscle & nerve 2010-06, Vol.41 (6), p.828-835
Main Authors: Melchionna, Roberta, Di Carlo, Anna, De Mori, Roberta, Cappuzzello, Claudia, Barberi, Laura, Musarò, Antonio, Cencioni, Chiara, Fujii, Nobutaka, Tamamura, Hirokazu, Crescenzi, Marco, Capogrossi, Maurizio C., Napolitano, Monica, Germani, Antonia
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Cycle - genetics
Cell Differentiation - drug effects
chemokine
Chemokine CXCL12 - genetics
Chemokine CXCL12 - pharmacology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Medical sciences
Mice
Myoblasts - cytology
Myoblasts - drug effects
myogenesis
Ophthalmology
Polymerase Chain Reaction
Receptors, CXCR - genetics
Receptors, CXCR4 - antagonists & inhibitors
Receptors, CXCR4 - genetics
regeneration
Retinopathies
RNA, Messenger - genetics
RNA, Small Interfering - genetics
satellite cells
SDF-1
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:The stromal cell–derived factor (SDF)‐1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution to postnatal myogenesis and the role of the alternate SDF‐1 receptor, CXC receptor 7 (CXCR7), are poorly characterized. Western blot analysis and real‐time polymerase chain reaction (PCR) were performed to evaluate in vitro the effect of SDF‐1 and CXCR4 and CXCR7 inhibition on myogenic differentiation. Proliferating myoblasts express CXCR4, CXCR7, and SDF‐1; during myogenic differentiation, CXCR4 and CXCR7 levels are downregulated, and SDF‐1 release is decreased. SDF‐1 anticipates myosin heavy chain accumulation and myotube formation in both C2C12 myoblasts and satellite cells. Interestingly, inhibition of CXCR4 and CXCR7 signaling, either by drugs or RNA interfererence, blocks myogenic differentiation. Further, the CXCR4 antagonist, 4F‐benzoyl‐TN14003, inhibits myoblast cell cycle withdrawal and decreases the retinoblastoma gene (pRb) product accumulation in its hypophosphorylated form. Our experiments demonstrate that SDF‐1 regulates myogenic differentiation via both CXCR4 and CXCR7 chemokine receptors. Muscle Nerve 000:000–000, 2010
ISSN:0148-639X
1097-4598
DOI:10.1002/mus.21611