Photodynamic chlorophyll a metabolites, including phytoporphyrin (phylloerythrin), in the blood of photosensitive livestock: Overview and measurement

AIM: To validate a spectrofluorometric method for measuring chlorophyll a metabolites, specifically phytoporphyrin (= phylloerythrin), as well as the chlorins, pheophorbide a and pyropheophorbide a, in the blood of photosensitive cattle and sheep. METHODS: Standard methanolic solutions of pheophorbi...

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Bibliographic Details
Published in:New Zealand veterinary journal 2010-06, Vol.58 (3), p.146-154
Main Authors: Campbell, WM, Dombroski, GS, Sharma, I, Partridge, AC, Collettt, MG
Format: Article
Language:English
Subjects:
Animals
Cattle
Chlorophyll
Chlorophyll - analogs & derivatives
Chlorophyll - blood
Chlorophyll - chemistry
Chlorophyll - metabolism
chromatography
Chromatography, High Pressure Liquid
Diseases
facial eczema
Fluorescent Antibody Technique
Livestock
Mass Spectrometry
Molecular Structure
pheophorbide
photosensitivity
Photosensitivity disorders
Photosensitivity Disorders - chemically induced
Photosensitivity Disorders - veterinary
phylloerythrin
phytoporphyrin
pyropheophorbide
spectrophotometry
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Summary:AIM: To validate a spectrofluorometric method for measuring chlorophyll a metabolites, specifically phytoporphyrin (= phylloerythrin), as well as the chlorins, pheophorbide a and pyropheophorbide a, in the blood of photosensitive cattle and sheep. METHODS: Standard methanolic solutions of pheophorbide a (25 µM), pyropheophorbide a (25 µM), and phytoporphyrin (
ISSN:0048-0169
1176-0710
1176-0710
DOI:10.1080/00480169.2010.67517