Polymorphic specificity of Q1/28, a monoclonal antibody that preferentially reacts with free class I heavy chains

The Q1/28 monoclonal antibody (mAb) reacts with class I HLA heavy chains, either when free or bound to beta sub(2)-microglobulin ( beta sub(2)-m). Subsequent to the initial description of Q1/28 by Quaranta and co-workers, the target epitope for the antibody was mapped to the alpha sub(3) domain of t...

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Bibliographic Details
Published in:Immunogenetics (New York) 1992-10, Vol.37 (1), p.73-76
Main Authors: BENJAMIN, R. J, ABRAMS, J. R, PARNES, J. R, ALEJANDRO MADRIGAL, J, PARHAM, P
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - genetics
Antibodies, Monoclonal - immunology
Antibody Specificity
Antigens
Biological and medical sciences
Cell Line
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histocompatibility antigens (hla, h-2 and other systems)
Histocompatibility Antigens Class I - genetics
Histocompatibility Antigens Class I - immunology
Humans
Isoelectric Focusing
Molecular immunology
Polymorphism, Genetic
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Description
Summary:The Q1/28 monoclonal antibody (mAb) reacts with class I HLA heavy chains, either when free or bound to beta sub(2)-microglobulin ( beta sub(2)-m). Subsequent to the initial description of Q1/28 by Quaranta and co-workers, the target epitope for the antibody was mapped to the alpha sub(3) domain of the class I heavy chain and found to be present on HLA-B7 but not HLA-A2 heavy chains. To further investigate the polymorphic specificity of Q1/28 we measured its binding to a panel of CIR cells which had been transfected with different class I HLA-A and B genes.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF00223548