Hemopoietic stem-cell harvesting and transplantation using G-CSF-primed BM: comparison with unprimed BM and G-CSF-primed PBSC

• Published in: Cytotherapy (Oxford, England) England), 1999-01, Vol.1 (5), p.409-416
• Main Authors: Lowenthal, R.M., Tuck, D., Tegg, E., Marsden, K.A., Rees, B., Luck, J., Ragg, S., Parker, N., Kotlovsky, N.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Bone Marrow Cells - cytology; bone marrow transplantation; Female; G-CSF; Granulocyte Colony-Stimulating Factor - metabolism; Hematopoietic Stem Cell Mobilization - methods; Hematopoietic Stem Cell Transplantation - methods; Humans; Male; Middle Aged; peripheral blood stem cells; Retrospective Studies; stem cells; Stem Cells - cytology; Time Factors; Treatment Outcome
• ISSN: 1465-3249; 1477-2566
• DOI: 10.1080/0032472031000141285

PBSC collected following G-CSF priming lead to more rapid hemopoietic reconstitution (HR) after autologous transplantation than do unprimed BMstem cells. However, PBSC have a number of disadvantages compared with BM cells, including the need for an extended collection period and requirement for good venous access. We retrospectively analysed our experience with an alternative source of hemopoietic stem cells, G-CSF primed BM. Fortyfour patients who underwent BM harvesting after 6 days' administration of G-CSF, at a dose of 5μg/kg per day, were compared with an equal number who underwent standard (unprimed) BM harvesting. We also analysed HR after autologous transplantation in 16 patients who received unprimed BM, 18 who received G-CSF-primed BM and 14 who received PBSC. G-CSF-primed BM was collected more quickly (p