Targeted delivery of antigens to the gut-associated lymphoid tissues: 2. Ex vivo evaluation of lectin-labelled albumin microspheres for targeted delivery of antigens to the M-cells of the Peyer's patches

The purpose of this study was to evaluate the possibility of lectin-coupled microspheres to improve the targeted delivery of protein antigens to the lymphoid tissues of mucosal surfaces. Bovine serum albumin containing acid phosphatase model protein and polystyrene microspheres were coupled with mou...

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Bibliographic Details
Published in:Journal of microencapsulation 2010, Vol.27 (4), p.325-336
Main Authors: Akande, Janet, Yeboah, Kwame G., Addo, Richard T., Siddig, Aladin, Oettinger, Carl W., D'Souza, Martin J.
Format: Article
Language:English
Subjects:
Acid Phosphatase - chemistry
Acid Phosphatase - pharmacology
Animals
Antigens - pharmacology
bioactivity of proteins
Biological and medical sciences
Cattle
Drug Delivery Systems
Gastrointestinal Tract - drug effects
General pharmacology
Intestinal Mucosa - drug effects
Lectins
Lectins - chemistry
Medical sciences
Mice
Mice, Inbred C57BL
Microspheres
Peyer's patches
Peyer's Patches - drug effects
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Plant Lectins - chemistry
Serum Albumin, Bovine - pharmacology
zeta potential
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Summary:The purpose of this study was to evaluate the possibility of lectin-coupled microspheres to improve the targeted delivery of protein antigens to the lymphoid tissues of mucosal surfaces. Bovine serum albumin containing acid phosphatase model protein and polystyrene microspheres were coupled with mouse M-cell-specific Ulex europaeus lectin. The coupling efficiency, physical characteristics and the binding capabilities of the microspheres to the follicle associated epithelium of the Peyer's patches were evaluated in vitro and ex vivo in mice intestine. The results showed that coupling of lectin to albumin microspheres did not significantly affect the bioactivity of the encapsulated acid phosphatase model protein. It was also shown that there was preferential binding of the lectin-coupled microspheres to the follicle-associated epithelium. It was concluded from the results of the study that coupling of ligands such as lectin specific to cells of the follicle associated epithelium can increase the targeting of encapsulated candidate antigens for delivery to the Peyer's patches of the intestine for improved oral delivery.
ISSN:0265-2048
1464-5246
DOI:10.3109/02652040903191834