Self-Controlled Monofunctionalization of Quantum Dots for Multiplexed Protein Tracking in Live Cells

Functionalization of quantum dots (QDs) was achieved by controlling the surface density of functional groups by electrostatic repulsion. These QDs were conjugated with His‐tagged proteins in vitro and in live cells by self‐assembly without requiring further fractionation, and dual‐color tracking of...

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Published in:Angewandte Chemie (International ed.) 2010-06, Vol.49 (24), p.4108-4112
Main Authors: You, Changjiang, Wilmes, Stephan, Beutel, Oliver, Löchte, Sara, Podoplelowa, Yulia, Roder, Friedrich, Richter, Christian, Seine, Thomas, Schaible, Dirk, Uzé, Gilles, Clarke, Samuel, Pinaud, Fabien, Dahan, Maxime, Piehler, Jacob
Format: Article
Language:English
Subjects:
fluorescence microscopy
HeLa Cells
Humans
Interferon-alpha - chemistry
Microscopy, Fluorescence
nanoparticles
Nitrilotriacetic Acid - chemistry
Protein Binding
protein-protein interactions
Quantum Dots
receptors
Staining and Labeling
Static Electricity
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Summary:Functionalization of quantum dots (QDs) was achieved by controlling the surface density of functional groups by electrostatic repulsion. These QDs were conjugated with His‐tagged proteins in vitro and in live cells by self‐assembly without requiring further fractionation, and dual‐color tracking of cell surface receptors was possible.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200907032