Selecting reference genes in the white blood cells of buffalos treated with recombinant growth hormone

In this study, we assessed the expression stability of eight genes (GADPH, ACTB, 18S, YWHAZ, SDHA, HMBS, SF3A1, and EEF1A) in the white blood cells of lactating buffalos and their possible use as reference genes for studies on growth hormone (GH)-treated animals. All of the genes showed acceptable s...

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Bibliographic Details
Published in:Analytical biochemistry 2010-08, Vol.403 (1-2), p.120-122
Main Authors: Castigliego, Lorenzo, Armani, Andrea, Li, Xiaoning, Grifoni, Goffredo, Gianfaldoni, Daniela, Guidi, Alessandra
Format: Article
Language:English
Subjects:
Animals
Buffaloes - genetics
Buffaloes - metabolism
Female
Gene Expression
Gene Expression Profiling
Genes
Growth Hormone - administration & dosage
Growth Hormone - metabolism
Lactation
Leukocytes - metabolism
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Summary:In this study, we assessed the expression stability of eight genes (GADPH, ACTB, 18S, YWHAZ, SDHA, HMBS, SF3A1, and EEF1A) in the white blood cells of lactating buffalos and their possible use as reference genes for studies on growth hormone (GH)-treated animals. All of the genes showed acceptable stability according to the threshold values suggested by some of the software that was used to analyze them, although the differences between the most stable (SF3A1 and ACTB) and the least stable (18S) were considerable. GH treatment did not influence their expression levels.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2010.04.001