DNA translocations contribute to chromosome length polymorphisms in Candida albicans

Rotating-gel electrophoresis and DNA hybridization were used to compare the electrophoretic karyotype of six Candida albicans isolates. The hybridization pattern for 22 cloned sequences, including eight previously unmapped genes, indicates that there are eight pair of homologous chromosomes in each...

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Bibliographic Details
Published in:Current genetics 1992-08, Vol.22 (2), p.93-100
Main Authors: Thrash-Bingham, C. (Bristol-Myers Squibb Pharmaceutical Research Inst., Princeton, NJ (USA). Dept. of Microbial Molecular Biology), Gorman, J.A
Format: Article
Language:English
Subjects:
Biological and medical sciences
CANDIDA ALBICANS
Candida albicans - genetics
CARIOTIPOS
CARTE GENETIQUE
CARYOTYPE
Chromosome Banding
CHROMOSOME TRANSLOCATION
Chromosomes, Fungal - ultrastructure
Cytogenetics
DNA translocations
DNA, Fungal - genetics
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
Electrophoresis, Gel, Pulsed-Field
Electrophoretic karyotype
Fundamental and applied biological sciences. Psychology
Gene mapping
Genes, Fungal
GENETIC MAPS
Genetic Markers
GENETIC POLYMORPHISM
Genetics of eukaryotes. Biological and molecular evolution
KARYOTYPES
Karyotyping - methods
MAPAS GENETICOS
Nucleic Acid Hybridization
POLIMORFISMO GENETICO
Polymorphism, Genetic
POLYMORPHISME GENETIQUE
Thallophyta, bryophyta
TRANSLOCACION DE CROMOSOMAS
TRANSLOCATION CHROMOSOMIQUE
Translocation, Genetic
Vegetals and fungi
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Description
Summary:Rotating-gel electrophoresis and DNA hybridization were used to compare the electrophoretic karyotype of six Candida albicans isolates. The hybridization pattern for 22 cloned sequences, including eight previously unmapped genes, indicates that there are eight pair of homologous chromosomes in each strain. However, since homologous chromosomes can differ in length, it is possible to resolve more than eight bands in some strains. The mapping data demonstrate that linkage groups are generally conserved suggesting that, in spite of gross karyotype differences, there is an underlying similarity in the genome organization of different isolates. The hybridization data also provide direct evidence that DNA translocations and reciprocal translocations contribute to chromosome length polymorphisms in C. albicans.
ISSN:0172-8083
1432-0983
DOI:10.1007/bf00351467