Screening of Chinese herbal medicines for antityrosinase activity in a cell free system and B16 cells

Tyrosinase inhibitors are becoming increasingly important in controlling skin hyperpigmentation. We aimed to screen 50 extracts from traditional Chinese medicines (TCM) for tyrosinase activity-inhibiting agents. The 50 herbal extracts were prepared from 32 herbs and 18 TCM formulas, which are used a...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2010-06, Vol.129 (3), p.387-390
Main Authors: Ye, Yan, Chou, Gui-Xin, Mu, Dan-Dan, Wang, Hui, Chu, Jian-Hong, Leung, Alexander Kai-Man, Fong, Wang-fun, Yu, Zhi-Ling
Format: Article
Language:English
Subjects:
Agaricales - enzymology
animal models
Animals
B16 melanoma cells
bioactive properties
Biological and medical sciences
cell growth
Cell Line, Tumor
Cell Survival - drug effects
Cosmetics
cytotoxicity
dosage
Drugs, Chinese Herbal - pharmacology
enzyme inhibition
enzyme inhibitors
Enzyme Inhibitors - pharmacology
General pharmacology
herbal medicines
hyperpigmentation
in vitro studies
inhibitory concentration 50
Medical sciences
medicinal plants
Medicine, Chinese Traditional
Mice
Monophenol Monooxygenase - antagonists & inhibitors
Mushroom tyrosinase
Oriental traditional medicine
oxidoreductases
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
pigmentation
plant extracts
screening
skin diseases
Skin Pigmentation - drug effects
skin whiteners
Skin whitening
Spectrophotometry
Traditional Chinese medicine
tyrosinase
tyrosinase inhibitors
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Summary:Tyrosinase inhibitors are becoming increasingly important in controlling skin hyperpigmentation. We aimed to screen 50 extracts from traditional Chinese medicines (TCM) for tyrosinase activity-inhibiting agents. The 50 herbal extracts were prepared from 32 herbs and 18 TCM formulas, which are used as folk skin whiteners in China and have not been investigated for their skin-whitening mechanisms. Each herb and formula was extracted with 30% ethanol and water, respectively, and followed by column chromatography for isolating bioactive substances such as saponins, flavonoids and alkaloids for the antityrosinase activity study. Every extract was tested using the cell free mushroom tyrosinase inhibitory assay at 2 mg/ml for the single herb extracts and 1 mg/ml for formula extracts. Extracts showing greater than 50% inhibition against mushroom tyrosinase activity were further examined by cellular tyrosinase assay in mouse B16 cells. The cytotoxicity in B16 cells was measured by methyl thiazolyl tetrazolium bromide (MTT) assay. In the cell-free assay, 10 out of the 50 extracts demonstrated more than 50% inhibition against mushroom tyrosinase activity. These 10 extracts were further assessed by cellular tyrosinase assay, and 6 showed > 50% inhibition with IC 50 values
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2010.04.009