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Development of Complete Homozygotes of Tobacco

True homozygotes of Nicotiana tabacum L., herein referred to as Ky Iso lines, are being developed from haploids. A technique is described whereby seedlings resulting from fertilization are eliminated, leaving only suspect haploids. After confirmation, haploids are treated to double the chromosomes a...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1963-09, Vol.141 (3586), p.1185-1186
Main Author: Stokes, G. W.
Format: Article
Language:English
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Summary:True homozygotes of Nicotiana tabacum L., herein referred to as Ky Iso lines, are being developed from haploids. A technique is described whereby seedlings resulting from fertilization are eliminated, leaving only suspect haploids. After confirmation, haploids are treated to double the chromosomes and these result in completely homozygous plants. One complete homozygote, Ky Iso 1 Ky 16, has been developed and shows extreme uniformity in all characters studied. The Ky Iso lines will be useful in genetic studies that require no plant-to-plant genotypic variability. The Ky Iso lines will be made available upon request as they are developed.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.141.3586.1185