Incorporation of Heterologous Deoxyribonucleic Acid into Mammalian Cells

Particles containing large molecular heterologous deoxyribonucleic acid have been incorporated into strain L cells in suspension tissue culture. The particles are Feulgen-positive, they are fluorescent under ultraviolet light when stained with acridine orange, and they appear in both the nucleus and...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1961-02, Vol.133 (3450), p.381-382
Main Authors: Bensch, Klaus G., King, Donald W.
Format: Article
Language:English
Subjects:
Acridines
Cell growth
Cell nucleus
Cytoplasm
DNA
Gelatins
L cells
Microscopy
Nucleic acids
Phenols
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Summary:Particles containing large molecular heterologous deoxyribonucleic acid have been incorporated into strain L cells in suspension tissue culture. The particles are Feulgen-positive, they are fluorescent under ultraviolet light when stained with acridine orange, and they appear in both the nucleus and cytoplasm in radioautographic preparations.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.133.3450.381