Immunohistochemical approach reveals involvement of inducible nitric oxide synthase in rat late development

To better understand the role of nitric oxide (NO) in mammal development, specifically in the transition of the fetal stages at birth, we studied the timing of cell-specific expression of inducible NO synthase (iNOS) isoform during gestational periods of rats, mainly at the late stages of intra-uter...

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Bibliographic Details
Published in:Journal of molecular histology 2009-06, Vol.40 (3), p.235-240
Main Authors: Zambuzzi, W. F, Paiva, K. B. S, Batista, A. C, Lara, V. S, Granjeiro, J. M
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Brief Communication
Cell Biology
Developmental Biology
Embryo, Mammalian - cytology
Embryo, Mammalian - enzymology
Embryonic Development
Female
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Immunohistochemistry - methods
Life Sciences
Nitric Oxide Synthase Type II - metabolism
Rats
Rats, Wistar
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Summary:To better understand the role of nitric oxide (NO) in mammal development, specifically in the transition of the fetal stages at birth, we studied the timing of cell-specific expression of inducible NO synthase (iNOS) isoform during gestational periods of rats, mainly at the late stages of intra-uterine development. Before experimentation, the samples were collected (from 17th to 21st gestational days), fixed in 10% buffered formalin and embedded in paraffin for histological procedures. Hereafter, the sections (5 μm thickness) obtained from different embryos were immunostained by avidin-biotin-immunoperoxidase technique, by using antibody against iNOS isoform. The most of cell immunopositive was suggestive of granulocyte-like cells and those cells were resident close to the blood vessels in different organs, such as: lung, liver or bone marrow environment. Sometimes we noted immunopositive cells in the blood flow, as reported in the thymus. In agreement, iNOS expression, obtained by western blotting analysis, showed the same profile. Together, our data shows that iNOS expression increased gradually during the late stages of rat development (from E17 to E21) and it was executed by cells close to blood vessels. Thus, we can clearly to predict that this expression was finely modulated and it contributes for time-line dependent NO production during rat late development.
ISSN:1567-2379
1567-2387
DOI:10.1007/s10735-009-9225-2