Transcriptional changes in CiFT-introduced transgenic trifoliate orange (Poncirus trifoliata L. Raf.)

Ectopic expression of a Flowering Locus T (CiFT) from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). To profile transcriptional effects of CiFT, mRNA extracted from the shoots of the transgenic trifoliate orange was subjected to genetic analysis using...

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Bibliographic Details
Published in:Tree physiology 2010-03, Vol.30 (3), p.431-439
Main Authors: Nishikawa, Fumie, Endo, Tomoko, Shimada, Takehiko, Fujii, Hiroshi, Shimizu, Tokurou, Kobayashi, Yasushi, Araki, Takashi, Omura, Mitsuo
Format: Article
Language:English
Subjects:
Flowers - physiology
Gene Expression Regulation, Plant - physiology
Plants, Genetically Modified
Poncirus - genetics
Poncirus - metabolism
Poncirus - physiology
Protein Array Analysis
Transcription, Genetic
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Summary:Ectopic expression of a Flowering Locus T (CiFT) from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). To profile transcriptional effects of CiFT, mRNA extracted from the shoots of the transgenic trifoliate orange was subjected to genetic analysis using a 22-K oligo DNA microarray. Microarray results and reverse transcription polymerase chain reaction analysis indicated that genes relating to transcription, cell wall modification and defense responses were expressed at higher levels in the transgenic shoots than in the control wild-type shoots. Of the genes showing ectopic mRNA accumulation, two SEPALLATA (SEP) and one FRUITFULL (FUL) homologues (CuSEP1, CuSEP3 or CuFUL) were introduced to Arabidopsis thaliana. Constitutive expression of each gene caused early flowering in transgenic Arabidopsis, suggesting that these genes could function as regulators of flowering time.
ISSN:0829-318X
1758-4469
DOI:10.1093/treephys/tpp122