Anti-angiogenic property of edible berry in a model of hemangioma

Hemangiomas represent a powerful model to study in vivo angiogenesis. Monocyte chemotactic protein 1 (MCP-1) is known to be responsible for recruiting macrophages to sites of infection or inflammation and facilitate angiogenesis. Recently we have demonstrated that edible berry extracts potently supp...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2003-06, Vol.544 (1), p.252-257
Main Authors: Atalay, Mustafa, Gordillo, Gayle, Roy, Sashwati, Rovin, Brad, Bagchi, Debasis, Bagchi, Manashi, Sen, Chandan K
Format: Article
Language:English
Subjects:
Angiogenesis Inhibitors - pharmacology
Animals
Antioxidant
Antioxidants - pharmacology
Autoantigens - biosynthesis
Blueberry Plants
Cell Survival
Chemokine CCL2
Dose-Response Relationship, Drug
Endothelium
Endothelium - drug effects
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fruit
Genes, Reporter
Hemangioma - drug therapy
Immunohistochemistry
Luciferases - metabolism
Mice
Monocyte chemotactic protein
NF-kappa B - metabolism
Nutrition
Plant Extracts - pharmacology
Time Factors
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hemangiomas represent a powerful model to study in vivo angiogenesis. Monocyte chemotactic protein 1 (MCP-1) is known to be responsible for recruiting macrophages to sites of infection or inflammation and facilitate angiogenesis. Recently we have demonstrated that edible berry extracts potently suppress inducible vascular endothelial growth factor expression and in vitro angiogenesis. Comparative analysis of several berry extracts led to the observation that wild blueberry and a berry mix were most effective. Our goal was to follow up on our findings with wild blueberry and the berry mix (OptiBerry). The present work rests on our current finding that these two berry powders significantly inhibit inducible MCP-1 expression in endothelioma cells. Therefore, we sought to examine the effects of wild blueberry and berry mix in an in vivo model of experimental angiogenesis. Reporter studies showed that the berry powders significantly inhibited basal MCP-1 transcription and inducible nuclear factor κB transcription. Endothelioma cells pre-treated with berry powders showed diminished ability to form hemangioma. Histological analysis demonstrated markedly decreased infiltration of macrophages in hemangioma of treated mice compared to placebo-treated controls. The current results provide the first in vivo evidence substantiating the anti-angiogenic property of edible berries.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)00509-X