Discovery of transposable element activity among progeny of tissue culture-derived maize plants

Tissue culture-derived plants of many species have often been observed to possess both genetic and cytogenetic abnormalities. A high frequency of structurally altered chromosomes in maize (Zea mays L.) plants regenerated from tissue culture led to the prediction that newly activated transposable ele...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1987-11, Vol.238 (4828), p.804-807
Main Authors: Peschke, Virginia M., Phillips, Ronald L., Gengenbach, Burle G.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Cell lines
Chromosome breakage
Chromosomes
Corn
cultivo de tejidos
culture de tissus
descendance
element chimique
elementos quimicos
elements
Embryos
Flowers & plants
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetic loci
Genetic transposition
Genetics
Genic rearrangement. Recombination. Transposable element
Molecular and cellular biology
Molecular genetics
Plant genetics
Plant tissue culture
Plants
progenie
progeny
regeneracion
regeneration
Reproduction
tissue culture
Tissue culture techniques
Translocation (Genetics)
Translocations (Genetics)
Transposons
Zea mays
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Summary:Tissue culture-derived plants of many species have often been observed to possess both genetic and cytogenetic abnormalities. A high frequency of structurally altered chromosomes in maize (Zea mays L.) plants regenerated from tissue culture led to the prediction that newly activated transposable elements could be detected in regenerated plants. Testcrosses of 1200 progeny from 301 regenerated maize plants confirmed that ten regenerated plants from two independent embryo cell lines contained an active Ac transposable element. No active Ac elements were present in the explant sources. Recovery of transposable element activity in regenerated plants indicates that some tissue culture-derived genetic variability may be the result of insertion or excision of transposable elements, or both.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.238.4828.804