Antigenic categorization of contemporary H3N2 Swine influenza virus isolates using a high-throughput serum neutralization assay

In vivo, neutralizing antibodies are critical for viral clearance. A high-throughput serum neutralization (HTSN) assay was developed to antigenically categorize Swine influenza virus (SIV) isolates. Uncategorized viruses were tested using a panel of antisera representing the H3N2 SIV subtypes and th...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2010-05, Vol.22 (3), p.352-359
Main Authors: Hause, Ben M, Oleson, Tracy A, Bey, Russell F, Stine, Douglas L, Simonson, Randy R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acids
Animals
antigenic variation
Antigens, Viral - blood
antiserum
Base Sequence
Bird Diseases - immunology
Bird Diseases - virology
Birds
blood serum
disease surveillance
Genes, Viral
Hemagglutination Inhibition Tests - veterinary
high-throughput serum neutralization
Humans
immunoassays
Influenza A virus
Influenza A Virus, H1N1 Subtype - immunology
Influenza A Virus, H3N2 Subtype - immunology
Influenza, Human - immunology
Molecular Sequence Data
neutralization tests
Neutralization Tests - veterinary
new methods
Orthomyxoviridae Infections - immunology
Orthomyxoviridae Infections - veterinary
pathogenicity
pathotypes
serodiagnosis
serotypes
Simian Immunodeficiency Virus - immunology
Swine
swine diseases
Swine Diseases - immunology
Swine Diseases - virology
vaccination
vaccine development
viral diseases of animals and humans
Viral Vaccines - immunology
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Summary:In vivo, neutralizing antibodies are critical for viral clearance. A high-throughput serum neutralization (HTSN) assay was developed to antigenically categorize Swine influenza virus (SIV) isolates. Uncategorized viruses were tested using a panel of antisera representing the H3N2 SIV subtypes and the results expressed as a serum neutralization ratio. Antisera were generated against contemporary isolates representing circulating H3N2 SIV subtypes (clusters I, III, IV). Reference viruses and the corresponding antisera were evaluated using traditional hemagglutination inhibition (HI) and the HTSN assays and good correlation (r = 0.84) was observed between the 2 tests. Categorical clustering of 40 recent (2008-2009) SIV isolates was assessed using the HTSN assay. The H3N2 SIV isolates with amino acid similarity >97% to the commonly used H3N2 cluster IV reference strain A/Swine/Ontario/33853/2005 (ON05) showed strong reactivity with cluster IV antisera. Isolates with
ISSN:1040-6387
1943-4936
DOI:10.1177/104063871002200302