Standardisation and establishment of a rabies ELISA test in European laboratories for assessing the efficacy of oral fox vaccination campaigns

A simple and rapid enzyme linked immunosorbent assay (ELISA) to detect rabies antibodies in field fox sera has been standardised and established in several European laboratories. The same panels of 100 coded sera were investigated by four laboratories using this ELISA assay and reference serum neutr...

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Bibliographic Details
Published in:Vaccine 2003-06, Vol.21 (21), p.2986-2993
Main Authors: Cliquet, Florence, Müller, Thomas, Mutinelli, Franco, Geronutti, Sabrina, Brochier, Bernard, Selhorst, Thomas, Schereffer, Jean-Luc, Krafft, Nicolas, Burow, Jeanette, Schameitat, Astrid, Schlüter, Hartmut, Aubert, Michel
Format: Article
Language:English
Subjects:
Administration, Oral
Agreements
Animals
Antibodies, Viral - blood
Biological and medical sciences
Cell culture
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Europe
Fluorescent Antibody Technique
Foxes - immunology
Fundamental and applied biological sciences. Psychology
Immunization
Laboratories
Laboratories - standards
Microbiology
Neutralization Tests
Rabies
Rabies - immunology
Rabies - prevention & control
Rabies - veterinary
Rabies Vaccines - administration & dosage
Rabies Vaccines - immunology
Reference Standards
Sensitivity and Specificity
Standardisation
Test systems
Vaccination - veterinary
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Virology
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Summary:A simple and rapid enzyme linked immunosorbent assay (ELISA) to detect rabies antibodies in field fox sera has been standardised and established in several European laboratories. The same panels of 100 coded sera were investigated by four laboratories using this ELISA assay and reference serum neutralisation techniques (fluorescent antibody virus neutralisation (FAVN) test and rapid fluorescent focus inhibition test (RFFIT)). This indirect ELISA technique is highly correlated with conventional seroneutralisation test on cell culture. Results obtained with this ELISA test provide an almost perfect agreement between laboratories while the agreement of FAVN test and RFFIT results is substantial and very satisfactory. In view of this study, this simple and rapid ELISA test is a suitable tool for evaluating the sero-conversion rate in fox populations following oral rabies vaccination campaigns in European countries.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00102-6