Radioprotective Effects of Ginsan, an Immunomodulator

• Published in: Radiation research 2003-06, Vol.159 (6), p.768-774
• Main Authors: Song, Jie-Young, Han, Seon-Kyu, Bae, Kang-Gyu, Lim, Dae-Seog, Son, Soo-Jung, Jung, In-Sung, Yi, Seh-Yoon, Yun, Yeon-Sook
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Biological and medical sciences; Bone marrow; Bone Marrow - drug effects; Bone Marrow - radiation effects; Bone marrow cells; Cell Survival - drug effects; Cell Survival - radiation effects; Cytokines; Cytokines - genetics; Female; Gamma Rays; Granulocyte macrophage progenitor cells; Hematopoiesis - drug effects; Hematopoiesis - radiation effects; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - radiation effects; Irradiation; Lymphocytes; Medical sciences; Mice; Mice, Inbred BALB C; Polysaccharides - pharmacology; Radiation dosage; Radiation-Protective Agents - pharmacology; REGULAR ARTICLES; RNA, Messenger - analysis; Space life sciences; Spleen; Spleen - drug effects; Spleen - radiation effects; Spleen cells
• ISSN: 0033-7587; 1938-5404
• DOI: 10.1667/0033-7587(2003)159[0768:REOGAI]2.0.CO;2

Song, J-Y., Han, S-K., Bae, K-G., Lim, D-S., Son, S-J., Jung, I-S., Yi, S-Y. and Yun, Y-S. Radioprotective Effects of Ginsan, an Immunomodulator. Radiat. Res. 159, 768–774 (2003). We previously reported that ginsan, a purified polysaccharide isolated from Panax ginseng, had a mitogenic activity, induced LAK cells, and increased levels of several cytokines. In an effort to identify other immunostimulatory effects, we evaluated the protective effects of ginsan injected in vivo against radiation by measuring its effects on the CFU-S bone marrow cells and spleen cells. Ginsan was found to significantly increase the number of bone marrow cells, spleen cells, granulocyte-macrophage colony-forming cells (GM-CFC), and circulating neutrophils, lymphocytes and platelets in irradiated mice. In addition, ginsan induced the endogenous production of cytokines such as Il1, Il6, Ifng and Il12, which are required for hematopoietic recovery, and was able to enhance Th1 function while interfering with the Th2 response in irradiated mice. We demonstrated that pretreatment with ginsan protected mice from the lethal effects of ionizing radiation more effectively than when it was given immediately after or at various times after irradiation. A significant increase in the LD50/30 from 7.54 Gy for PBS injection to 10.93 Gy for mice pretreated with 100 mg/kg ginsan was observed. These findings indicate that ginsan may be a useful agent to reduce the time necessary for reconstituting hematopoietic cells after irradiation.