Science Letters： Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis

• Published in: Journal of Zhejiang University. B. Science 2010-03, Vol.11 (3), p.221-226
• Main Authors: Tan, Xu-fei, Chen, Feng, Wu, Shan-shan, Shi, Yu, Liu, Dong-cheng, Chen, Zhi
• Format: Article
• Language: English
• Subjects: Animals; Biomarkers - analysis; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - metabolism; Concanavalin A; Disease Models, Animal; Gene Expression Profiling; Gene Expression Regulation - drug effects; Humans; Liver - drug effects; Liver - metabolism; Male; Mice; Mice, Inbred BALB C; PCR检测; Proteome - analysis; 差异表达; 蛋白A; 蛋白质组分
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.B0900351

Objective： To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A （Con A）-induced hepatitis. Methods： Twenty-five mice were randomly divided into five groups： an untreated group, a control group injected with phosphate buffered saline （PBS）, and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel electrophoresis （2-DE） and mass spectrometry （MS） were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction （qRT-PCR） was performed to verify the results. Results： In mice with Con A-induced hepatitis, expression levels of four proteins were increased： RIKEN, fructose bisphosphatase 1 （fbp1）, ketohexokinase （khk）, and Chain A of class pi glutathione S-transferase. Changes in fbpl and khk were confirmed by qRT-PCR. Conclusion： Levels of two proteins, fbp1 and khk, are clearly up-regulated in mice with Con A-induced hepatitis.