Phytoplankton optical properties: flow cytometric examinations of dilution-induced effects

Flow cytometry is used to measure dilution-induced changes in the optical properties of Dunaliella tertiolecta-light scatter, cell fluorescence, and refractive index. Observed changes in cell optical properties are compared to simultaneous measures of cell volume and count, extracted chlorophyll a c...

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Bibliographic Details
Published in:Applied optics (2004) 1988-04, Vol.27 (7), p.1262-1269
Main Authors: Ackleson, S G, Spinrad, R W, Yentsch, C M, Brown, J, Korjeff-Bellows, W
Format: Article
Language:English
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Summary:Flow cytometry is used to measure dilution-induced changes in the optical properties of Dunaliella tertiolecta-light scatter, cell fluorescence, and refractive index. Observed changes in cell optical properties are compared to simultaneous measures of cell volume and count, extracted chlorophyll a concentration, and the (14)C uptake rate. Flow cytometric measurements reveal short-term dilution effects (within 1 h of dilution) that, are not evident in other morphological or physiological measurements such as cell volume, extracted chlorophyll a concentration, and (14)C uptake rate. Data are presented which suggest that these short-term changes in cell optical properties are the result of changes in the real component of refractive index, possibly due to a rapid and temporary rearrangement of the internal cellular structure. Long-term changes are observed in time series measurements of cell volume and count, extracted chlorophyll a concentration, and (14)C uptake rate.
ISSN:1559-128X
DOI:10.1364/AO.27.001262