UV-Induced Grafting Processes with In Situ Formed Photomask for Micropatterning of Two-Component Biomolecules

We report a photolithographic process for micropatterning of two-component biomolecules on a transparent organic film via lateral functional polymer brushes of poly(sodium acrylate) (P(AA)) and poly(glycidyl methacrylate) (P(GMA)). The pattern of binary polymer brushes were prepared via consecutive...

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Bibliographic Details
Published in:Langmuir 2010-06, Vol.26 (12), p.9905-9910
Main Authors: Tang, S. C, Xie, J. Y, Huang, Z. H, Xu, F. J, Yang, Wantai
Format: Article
Language:English
Subjects:
Antibodies, Anti-Idiotypic
Avidin
Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials
Biotin - chemistry
Chemistry
Exact sciences and technology
General and physical chemistry
Immunoglobulin G - chemistry
Molecular Imprinting - methods
Polymers - chemistry
Ultraviolet Rays
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Summary:We report a photolithographic process for micropatterning of two-component biomolecules on a transparent organic film via lateral functional polymer brushes of poly(sodium acrylate) (P(AA)) and poly(glycidyl methacrylate) (P(GMA)). The pattern of binary polymer brushes were prepared via consecutive UV-initiated grafting processes, under the assistance of the in situ formed poly (4,4′-bi[N-(4-vinylbenzyl) pyridinium]) (P(BVV)) photomask. The epoxy groups of the P(GMA) microdomains can be aminated for covalently coupling biotin, while the P(AA) microdomains were used for immobilizing immunoglobulin (IgG). The resulting biotin- and IgG-coupled microdomains interact specifically with their corresponding target proteins, avidin and anti-IgG, respectively.
ISSN:0743-7463
1520-5827
DOI:10.1021/la100344f