Frequent polymorphism in the 13th exon of the adenomatous polyposis coli gene

We have studied the DNA from 170 individuals affected with familial adenomatous polyposis and from 20 uneffected individuals. Denaturing gradient gel electrophoresis of polymerase chain reaction amplified DNA from the adenomatous polyposis coli (APC) gene demonstrated three major patterns consistent...

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Bibliographic Details
Published in:Human genetics 1992-09, Vol.90 (1-2), p.161-163
Main Authors: OLSCHWANG, S, LAURENT-PUIG, P, THUILLE, B, THOMAS, G
Format: Article
Language:English
Subjects:
Adenomatous Polyposis Coli - genetics
Base Sequence
Biological and medical sciences
Electrophoresis
Exons - genetics
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Medical sciences
Molecular Sequence Data
Oligonucleotide Probes - genetics
Polymerase Chain Reaction
Polymorphism, Genetic - genetics
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Citations: Items that cite this one
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Summary:We have studied the DNA from 170 individuals affected with familial adenomatous polyposis and from 20 uneffected individuals. Denaturing gradient gel electrophoresis of polymerase chain reaction amplified DNA from the adenomatous polyposis coli (APC) gene demonstrated three major patterns consistent with the existence of two different sequences in exon 13 of the gene in the human population. Direct sequencing of the amplified product from DNAs producing these three patterns confirmed the presence of a polymorphism in the coding region of the APC gene.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00210765