Monitoring and modeling density-dependent growth of anchorage-dependent cells

The density‐dependent growth of Chinese hamster ovary (CHO) cells was monitored on‐line by using an inverted microscope. A flow system was employed for cell cultivation so that nutrient concentration could be maintained and metabolic wastes were removed. With the help of video image analysis, local...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1993-02, Vol.41 (3), p.380-389
Main Authors: Ruaan, R.-C., Tsai, G.-J., Tsao, G. T.
Format: Article
Language:English
Subjects:
anchorage-dependent cells
Animal cells
Biological and medical sciences
Biotechnology
CHO cells
density-dependent growth
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
image analysis
Methods. Procedures. Technologies
Miscellaneous
model simulation
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Summary:The density‐dependent growth of Chinese hamster ovary (CHO) cells was monitored on‐line by using an inverted microscope. A flow system was employed for cell cultivation so that nutrient concentration could be maintained and metabolic wastes were removed. With the help of video image analysis, local cells density could be accurately calculated and cell motility and exposed cell surface area could be estimated. A computer program which accounted for change of sell size and translocation of cells was developed to stimulate dell growth. The stimulated results of the population dynamics and the variations in cell size showed good agreement with our experimental observations, Cell motility and initial cell distribution on the substratum were found to have strong effect on cell growth. © 1993 John Wiley & Sons, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260410313