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Preferential Cleavage of N−N Hydrazone Bonds for Sequencing Bis-arylhydrazone Conjugated Peptides by Electron Transfer Dissociation
Electron transfer dissociation (ETD) was used to sequence bis-arylhydrazone (BAH)-cross-linked peptides through preferential cleavage of the hydrazone bond. On average, 58% of the observed ETD product ion abundance was accounted for by fragment ions due to selective cleavage of the N12−N13 hydrazone...
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Published in: | Analytical chemistry (Washington) 2010-07, Vol.82 (13), p.5751-5759 |
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Main Authors: | , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Electron transfer dissociation (ETD) was used to sequence bis-arylhydrazone (BAH)-cross-linked peptides through preferential cleavage of the hydrazone bond. On average, 58% of the observed ETD product ion abundance was accounted for by fragment ions due to selective cleavage of the N12−N13 hydrazone bond. Dissociation of the N12−N13 hydrazone bond yielded the two constituent peptides, one an even-electron product ion termed Lα12, the other an odd-electron radical ion termed Lβ11•, which allowed each peptide to be individually sequenced by MS/MS methods and the site of cross-linking to be identified. The proposed pathway for the dissociation of the hydrazone bond involves transfer of the electron directly to the protonated hydrazone functionality and subsequent rearrangement to yield the Lα12 and Lβ11• products. Collision induced dissociation (CID) of the even-electron Lα12 product yielded a series of b- and y-type ions; CID of the odd-electron Lβ11• product resulted in a wide range of fragment ions including a-, b-, c-, y-, and z-type ions. |
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ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/ac100788a |