Liquid Chromatography-Tandem Mass Spectrometry Assay for Androstenedione, Dehydroepiandrosterone, and Testosterone with Pediatric and Adult Reference Intervals

Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedione, dehydroepiandrosterone (DHEA), and testosterone in these pa...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2010-07, Vol.56 (7), p.1138-1147
Main Authors: KUSHNIR, Mark M, BLAMIRES, Takara, ROCKWOOD, Alan L, ROBERTS, William L, BINGFANG YUE, ERDOGAN, Evrim, BUNKER, Ashley M, WAYNE MEIKLE, A
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Analytical, structural and metabolic biochemistry
Androgens
Androstenedione - blood
Biological and medical sciences
Child
Child, Preschool
Children
Chromatography
Chromatography, Liquid
Dehydroepiandrosterone - blood
Female
Fundamental and applied biological sciences. Psychology
Humans
Infant
Investigative techniques, diagnostic techniques (general aspects)
Liquid chromatography
Male
Mass spectrometry
Medical sciences
Middle Aged
Molecular biophysics
Older people
Reference Values
Sensitivity and Specificity
Tandem Mass Spectrometry
Testosterone - blood
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Summary:Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedione, dehydroepiandrosterone (DHEA), and testosterone in these patients. We spiked 200 muL of serum or plasma with isotope-labeled internal standards and performed extraction with methyl t-butyl ether. We then derivatized the extracts with hydroxylamine and analyzed them by LC-MS/MS using a 2-dimensional chromatographic separation with a 3.5-min analysis time. Total imprecision for each analyte was
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2010.143222