Plasma MicroRNA 499 as a Biomarker of Acute Myocardial Infarction

MicroRNAs (miRNAs) are endogenous small RNAs 21-25 nucleotides in length. Recently, we reported that miRNA 208 (miR-208) is produced exclusively in the rat myocardium and that plasma miR-208 is a biomarker of myocardial injury in rats. In the present study, we assessed the hypothesis that plasma con...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2010-07, Vol.56 (7), p.1183-1185
Main Authors: ADACHI, Taichi, NAKANISHI, Michio, OTSUKA, Yoritaka, NISHIMURA, Kunihiro, HIROKAWA, Gou, GOTO, Yoichi, NONOGI, Hiroshi, IWAI, Naoharu
Format: Article
Language:English
Subjects:
Acute coronary syndromes
Adult
Aged
Analytical, structural and metabolic biochemistry
Angina pectoris
Biological and medical sciences
Biomarkers
Biomarkers - blood
Cardiovascular diseases
Female
Fundamental and applied biological sciences. Psychology
Heart attacks
Heart failure
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical imaging
Medical sciences
MicroRNAs - blood
Middle Aged
Molecular biophysics
Myocardial infarction
Myocardial Infarction - blood
Myocardial Infarction - diagnosis
Myocardium - metabolism
Plasma
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Summary:MicroRNAs (miRNAs) are endogenous small RNAs 21-25 nucleotides in length. Recently, we reported that miRNA 208 (miR-208) is produced exclusively in the rat myocardium and that plasma miR-208 is a biomarker of myocardial injury in rats. In the present study, we assessed the hypothesis that plasma concentrations of myocardial-specific miRNAs can be used to diagnose myocardial injury in humans. We used array analysis of miRNA production in various human tissues to identify heart-specific miRNAs. We assessed the plasma concentrations of miR-499 in 14 individuals with acute coronary syndromes, 15 individuals with congestive heart failure, and 10 individuals without cardiovascular diseases. Plasma miR-499 concentrations were measured with a real-time reverse-transcription PCR method that used an artificial small RNA as an internal calibrator. The miRNA array analysis of various human tissues indicated that miR-499 was produced almost exclusively in the heart. Plasma miR-499 concentrations were measurably increased in all individuals with acute myocardial infarction but were below the limit of detection for all individuals in the other patient groups. The plasma concentration of miR-499 may be a useful biomarker of myocardial infarction in humans.
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2010.144121