Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor

We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly( N , N -dimethylacrylamide)-based support, nonselective ion-exchange type behaviour between the opp...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2010-01, Vol.8 (1), p.2414-2418
Main Authors: Besenius, Pol, Cormack, Peter A. G, Ludlow, R. Frederick, Otto, Sijbren, Sherrington, David C
Format: Article
Language:English
Subjects:
Acrylamides - chemistry
Amantadine - chemistry
Chromatography, Affinity
Chromatography, High Pressure Liquid
Combinatorial Chemistry Techniques - methods
Disulfides - chemistry
Nanostructures - chemistry
Water - chemistry
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Summary:We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly( N , N -dimethylacrylamide)-based support, nonselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular recognition arising in aqueous solution reactions is manifest also in the analogous templated solid phase DCL syntheses. The ability of a polymer bound template to identify and isolate a synthetic receptor via dynamic combinatorial chemistry was not compromised by the large size of the library, consisting of well over 140 theoretical members, demonstrating the practical advantages of a polymer-supported DCL methodology. We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library consisting of well over 140 theoretical members using a polymer-bound template.
ISSN:1477-0520
1477-0539
DOI:10.1039/c000333f